Expression, crystallization and preliminary X-ray crystallographic analysis of peptide deformylase from Xanthomonas oryzae pv. oryzae

摘要

Peptide deformylase (PDF) catalyzes the removal of the N-formyl group from the N-terminus of nascent polypeptides. This process is essential for the survival of bacteria. As it is an antibacterial drug target, PDF from X. oryzae pv. oryzae was cloned, purified and crystallized for preliminary X-ray studies.